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Promega gst pull-downs pgex4t-1
Gst Pull Downs Pgex4t 1, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+pull-downs+pgex4t-1/pgex+vectors/pmc02546611-83-119-137
Average 90 stars, based on 1 article reviews
gst pull-downs pgex4t-1 - by Bioz Stars, 2026-10
90/100 stars

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Western Blot:

Article Title: Nervous Wreck and Cdc42 cooperate to regulate endocytic actin assembly during synaptic growth
Article Snippet: A fragment of Nwk encoding amino acids 630-754 and encompassing SH3b was used to immunize rabbits (Invitrogen, Carlsbad, CA) to generate α-Nwk antibody #69009, which was used for immunoblots ( ). α-Nwk antibody #970 ( Coyle et al., 2004 ) was used for immunoblots and immunohistochemistry (Figs. and ). α-Dynamin, α-Dap160 ( Roos and Kelly, 1998 ), and α-Rab11 ( Khodosh et al., 2006 ) antibodies have been described previously. fig ft0 fig mode=article f1 Figure 4 caption a4 caption a8 Expression of Nwk SH3 domain mutants in vivo fig ft0 fig mode=article f1 Figure 8 caption a4 caption a8 Cooperation of Nwk and Cdc42 in synaptic growth GST pull-downs, immunoprecipitations, and yeast two-hybrid assays For GST pull-downs, a fragment of Nwk encoding amino acids 1-754 or SH3 domain variants W531A and W677A was cloned into pGEX4T-1 (Promega, Madison, WI).

Immunohistochemistry:

Article Title: Nervous Wreck and Cdc42 cooperate to regulate endocytic actin assembly during synaptic growth
Article Snippet: A fragment of Nwk encoding amino acids 630-754 and encompassing SH3b was used to immunize rabbits (Invitrogen, Carlsbad, CA) to generate α-Nwk antibody #69009, which was used for immunoblots ( ). α-Nwk antibody #970 ( Coyle et al., 2004 ) was used for immunoblots and immunohistochemistry (Figs. and ). α-Dynamin, α-Dap160 ( Roos and Kelly, 1998 ), and α-Rab11 ( Khodosh et al., 2006 ) antibodies have been described previously. fig ft0 fig mode=article f1 Figure 4 caption a4 caption a8 Expression of Nwk SH3 domain mutants in vivo fig ft0 fig mode=article f1 Figure 8 caption a4 caption a8 Cooperation of Nwk and Cdc42 in synaptic growth GST pull-downs, immunoprecipitations, and yeast two-hybrid assays For GST pull-downs, a fragment of Nwk encoding amino acids 1-754 or SH3 domain variants W531A and W677A was cloned into pGEX4T-1 (Promega, Madison, WI).

Expressing:

Article Title: Nervous Wreck and Cdc42 cooperate to regulate endocytic actin assembly during synaptic growth
Article Snippet: A fragment of Nwk encoding amino acids 630-754 and encompassing SH3b was used to immunize rabbits (Invitrogen, Carlsbad, CA) to generate α-Nwk antibody #69009, which was used for immunoblots ( ). α-Nwk antibody #970 ( Coyle et al., 2004 ) was used for immunoblots and immunohistochemistry (Figs. and ). α-Dynamin, α-Dap160 ( Roos and Kelly, 1998 ), and α-Rab11 ( Khodosh et al., 2006 ) antibodies have been described previously. fig ft0 fig mode=article f1 Figure 4 caption a4 caption a8 Expression of Nwk SH3 domain mutants in vivo fig ft0 fig mode=article f1 Figure 8 caption a4 caption a8 Cooperation of Nwk and Cdc42 in synaptic growth GST pull-downs, immunoprecipitations, and yeast two-hybrid assays For GST pull-downs, a fragment of Nwk encoding amino acids 1-754 or SH3 domain variants W531A and W677A was cloned into pGEX4T-1 (Promega, Madison, WI).

In Vivo:

Article Title: Nervous Wreck and Cdc42 cooperate to regulate endocytic actin assembly during synaptic growth
Article Snippet: A fragment of Nwk encoding amino acids 630-754 and encompassing SH3b was used to immunize rabbits (Invitrogen, Carlsbad, CA) to generate α-Nwk antibody #69009, which was used for immunoblots ( ). α-Nwk antibody #970 ( Coyle et al., 2004 ) was used for immunoblots and immunohistochemistry (Figs. and ). α-Dynamin, α-Dap160 ( Roos and Kelly, 1998 ), and α-Rab11 ( Khodosh et al., 2006 ) antibodies have been described previously. fig ft0 fig mode=article f1 Figure 4 caption a4 caption a8 Expression of Nwk SH3 domain mutants in vivo fig ft0 fig mode=article f1 Figure 8 caption a4 caption a8 Cooperation of Nwk and Cdc42 in synaptic growth GST pull-downs, immunoprecipitations, and yeast two-hybrid assays For GST pull-downs, a fragment of Nwk encoding amino acids 1-754 or SH3 domain variants W531A and W677A was cloned into pGEX4T-1 (Promega, Madison, WI).

Clone Assay:

Article Title: Nervous Wreck and Cdc42 cooperate to regulate endocytic actin assembly during synaptic growth
Article Snippet: A fragment of Nwk encoding amino acids 630-754 and encompassing SH3b was used to immunize rabbits (Invitrogen, Carlsbad, CA) to generate α-Nwk antibody #69009, which was used for immunoblots ( ). α-Nwk antibody #970 ( Coyle et al., 2004 ) was used for immunoblots and immunohistochemistry (Figs. and ). α-Dynamin, α-Dap160 ( Roos and Kelly, 1998 ), and α-Rab11 ( Khodosh et al., 2006 ) antibodies have been described previously. fig ft0 fig mode=article f1 Figure 4 caption a4 caption a8 Expression of Nwk SH3 domain mutants in vivo fig ft0 fig mode=article f1 Figure 8 caption a4 caption a8 Cooperation of Nwk and Cdc42 in synaptic growth GST pull-downs, immunoprecipitations, and yeast two-hybrid assays For GST pull-downs, a fragment of Nwk encoding amino acids 1-754 or SH3 domain variants W531A and W677A was cloned into pGEX4T-1 (Promega, Madison, WI).

Generated:

Article Title: Nervous Wreck and Cdc42 cooperate to regulate endocytic actin assembly during synaptic growth
Article Snippet: A fragment of Nwk encoding amino acids 630-754 and encompassing SH3b was used to immunize rabbits (Invitrogen, Carlsbad, CA) to generate α-Nwk antibody #69009, which was used for immunoblots ( ). α-Nwk antibody #970 ( Coyle et al., 2004 ) was used for immunoblots and immunohistochemistry (Figs. and ). α-Dynamin, α-Dap160 ( Roos and Kelly, 1998 ), and α-Rab11 ( Khodosh et al., 2006 ) antibodies have been described previously. fig ft0 fig mode=article f1 Figure 4 caption a4 caption a8 Expression of Nwk SH3 domain mutants in vivo fig ft0 fig mode=article f1 Figure 8 caption a4 caption a8 Cooperation of Nwk and Cdc42 in synaptic growth GST pull-downs, immunoprecipitations, and yeast two-hybrid assays For GST pull-downs, a fragment of Nwk encoding amino acids 1-754 or SH3 domain variants W531A and W677A was cloned into pGEX4T-1 (Promega, Madison, WI).

Cloning:

Article Title: Nervous Wreck and Cdc42 cooperate to regulate endocytic actin assembly during synaptic growth
Article Snippet: A fragment of Nwk encoding amino acids 630-754 and encompassing SH3b was used to immunize rabbits (Invitrogen, Carlsbad, CA) to generate α-Nwk antibody #69009, which was used for immunoblots ( ). α-Nwk antibody #970 ( Coyle et al., 2004 ) was used for immunoblots and immunohistochemistry (Figs. and ). α-Dynamin, α-Dap160 ( Roos and Kelly, 1998 ), and α-Rab11 ( Khodosh et al., 2006 ) antibodies have been described previously. fig ft0 fig mode=article f1 Figure 4 caption a4 caption a8 Expression of Nwk SH3 domain mutants in vivo fig ft0 fig mode=article f1 Figure 8 caption a4 caption a8 Cooperation of Nwk and Cdc42 in synaptic growth GST pull-downs, immunoprecipitations, and yeast two-hybrid assays For GST pull-downs, a fragment of Nwk encoding amino acids 1-754 or SH3 domain variants W531A and W677A was cloned into pGEX4T-1 (Promega, Madison, WI).



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Promega gst pull-downs pgex4t-1
Gst Pull Downs Pgex4t 1, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+pull-downs+pgex4t-1/pgex+vectors/pmc02546611-83-119-137
Average 90 stars, based on 1 article reviews
gst pull-downs pgex4t-1 - by Bioz Stars, 2026-10
90/100 stars
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